Enhancing recombinant protein production through Cre-loxP mediated chromosomal rearrangement evolution in Kluyveromyces marxianus.

ABSTRACT: Chromosomal rearrangements can drive adaptive evolution; however, whether the rearrangements in non-coding and non-promoter regions can lead to new phenotypes under selective pressures remains unclear. Additionally, highly producing recombinant proteins is a key industrial task but poses stress on host cells. Therefore, it is desirable to investigate the role of chromosomal rearrangement in non-coding and non-promoter regions during high-yield recombinant protein phenotype formation. In this study, we utilize the Kluyveromyces marxianus strain as the host for recombinant protein production, with the recombinant fusion protein comprising leghemoglobin (LBA) and enhanced green fluorescent protein serving as a reporter. Iterative evolution is conducted to select high-yield strains using Cre-l

SUBMITTER: Ren H 

PROVIDER: S-EPMC12038034 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature