Circular RNAs increase during vascular cell differentiation and are biomarkers for vascular disease.

ABSTRACT:

Aims

The role of circular RNAs (circRNAs) and their regulation in health and disease are poorly understood. Here, we systematically investigated the temporally resolved transcriptomic expression of circRNAs during differentiation of human induced pluripotent stem cells (iPSCs) into vascular endothelial cells (ECs) and smooth muscle cells (SMCs) and explored their potential as biomarkers for human vascular disease.

Methods and results

Using high-throughput RNA sequencing and a de novo circRNA detection pipeline, we quantified the daily levels of 31 369 circRNAs in a 2-week differentiation trajectory from human stem cells to proliferating mesoderm progenitors to quiescent, differentiated EC and SMC. We detected a significant global increase in RNA circularization, with 397 and

SUBMITTER: Northoff BH 

PROVIDER: S-EPMC12038242 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature