Next-Gen Point-of-Care Tool for Ultra-Sensitive Detection of Urinary Spermine for Prostate Cancer Diagnosis.

ABSTRACT: Prostate cancer (PCa), the second most common cancer in men, demands effective early detection strategies. Elevated spermine levels in the prostate tissue contrast with decreased urinary concentrations in PCa patients. Here, we present a novel sensing platform combining differential pulse voltammetry and an extended gate field-effect transistor (EGFET) with a molecularly imprinted polymer|molecular imprinting (MIP) nanofilm for selective and sensitive spermine detection. Key advancements include successfully constructing and characterizing a pseudoreference electrode and a precisely engineered analyte binding interface. The Ag/AgCl pseudoreference electrode exhibited high reliability and reproducibility, optimized to enhance conductivity and minimize interference noises. Electrochemical an

SUBMITTER: Dehghani P 

PROVIDER: S-EPMC12038882 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature