Characterization of Cell Surface Glycoproteins Using Enzymatic Treatment and Mass Spectrometry.

ABSTRACT: Almost all proteins on the cell surface are modified by glycosylation. Cell surface glycoproteins participate in various cellular pathways, such as cell adhesion, cell-cell communication, and immune response. Due to their functional importance, glycoproteins on the cell surface often serve as potential therapeutic targets. Recent advancements in mass spectrometry (MS) have facilitated the characterization of glycoproteins that are generally localized on the cell surface, secreted to the extracellular environment, or found in intracellular organelles such as the endoplasmic reticulum, Golgi apparatus, and peroxisome. However, the selective characterization of glycoproteins on the cell surface remains challenging. In this study, we applied enzymatic treatment to live cells, followed by MS-ba

SUBMITTER: Lin DC 

PROVIDER: S-EPMC12038886 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature