NF-κB-mediated enhancement of H3K27me3 through EZH2: a mechanism to suppress pyocyanin-induced autophagy in macrophages.

ABSTRACT:

Background

Histone modification is a key mechanism of epigenetic regulation. Our previous study demonstrated that histone H3 acetylation at lysine 27 (H3K27ac) promotes pyocyanin (PYO)-induced autophagy in macrophages. However, the regulatory role of H3K27 trimethylation (H3K27me3) in this process remains unclear.

Methods

THP-1 macrophages were treated with PYO, and autophagy was assessed by evaluating LC3B II expression and autophagosome formation. The expression of EZH2 and JMJD3 was analyzed to identify the key enzyme responsible for regulating H3K27me3. Nuclear-cytoplasmic fractionation and co-immunoprecipitation were performed to determine the distribution of NF-κB and its interaction with H3K27me3. To explore the role of H3K27me3 in PYO-induced autophagy, cells were co

SUBMITTER: Wang J 

PROVIDER: S-EPMC12038963 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature