Development of a triplex TaqMan probe-based real-time PCR assay for simultaneous detection of swine influenza virus, porcine reproductive and respiratory syndrome virus, and porcine circovirus type 2.

ABSTRACT:

Background

Porcine respiratory disease complex poses a substantial economic challenge for the swine farms due to its prevalence in pig farming environments. The disease is primarily caused by viral pathogens such as swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus type 2 (PCV2). Given the high incidence and morbidity associated with these viruses, effective control strategies rely on rapid and accurate diagnosis.

Results

To this end, we developed and validated a triplex TaqMan probe-based real-time PCR assay for the simultaneous detection and differentiation of SIV, PRRSV, and PCV2. This assay demonstrated high specificity, with no observed cross-reactivity between the target viruses. The assay's sensitivity was

SUBMITTER: Wang M 

PROVIDER: S-EPMC12038986 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature