A Self-Assembly Pipette Tip Restricted Access Mesoporous Polypyrrole Solid-Phase Extraction Coupled With Capillary Electrophoresis With Diode Array Detection for the Determination of Enalapril in Urine Samples.

ABSTRACT: A miniaturized self-assembly pipette tip with restricted access mesoporous polypyrrole solid-phase extraction, combined with capillary electrophoresis with diode array detection (CE-DAD), was developed to rapidly extract and determine enalapril from urine samples. The CE-DAD technique used 50 mmol L^-1 phosphate (pH 7) as the background electrolyte, a voltage of 13 kV, a 30 mbar hydrodynamic injection for 4 s, a capillary temperature of 25°C, and a wavelength of 195 nm to achieve a migration time of 6.3 min with satisfactory peak asymmetry and no interfering and/or baseline noise. The factors that influenced the extraction efficiency were evaluated and optimized: 750 µL sample at pH 7.5, 40 mg adsorbent, 250 µL hexane as a washing solvent, and 750 µL acetonitrile as eluent, resul

SUBMITTER: Carvalho IA 

PROVIDER: S-EPMC12039168 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature