PtWAVE: a high-sensitive deconvolution software of sequencing trace for the detection of large indels in genome editing.

ABSTRACT:

Background

Tracking of Insertions and DEletions (TIDE) analysis, which computationally deconvolves capillary sequencing data derived from the DNA of bulk or clonal cell populations to estimate the efficiency of targeted mutagenesis by programmable nucleases, has played a significant role in the field of genome editing. However, the detection range covered by conventional TIDE analysis is limited. Range extension for deconvolution is required to detect larger deletions and insertions (indels) derived from genome editing in TIDE analysis. However, extending the deconvolution range introduces uncertainty into the deconvolution process. Moreover, the accuracy and sensitivity of TIDE analysis tools for large deletions (> 50 bp) remain poorly understood.

Results

In this study, we

SUBMITTER: Nakamae K 

PROVIDER: S-EPMC12039204 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature