Rapid <i>in vitro</i> evolution of flucytosine resistance in <i>Candida auris</i>.

ABSTRACT: The pan-antifungal-resistant pathogen Candida auris has been causing high-mortality infection outbreaks in hospitals and healthcare settings. The prodrug 5-fluorocytosine (5FC) is one of four chemical entities, but its clinical use as an antifungal drug has been limited owing to pronounced resistance. However, antifungal combination therapy with 5FC appears as a promising strategy for treating C. auris infections. Here, we show that a C. auris clinical isolate can rapidly acquire genetic mutations to mount 5FC resistance after only one to two passages under drug selection. We exploit a new bioinformatics workflow to identify genetic polymorphisms from RNA-seq data. Strikingly, we identify several mutations in the FUR1 gene encoding the 5-fluorouracil convertase

SUBMITTER: Phan-Canh T 

PROVIDER: S-EPMC12039228 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature