Co-carriage of diverse vancomycin-resistant <i>Enterococcus faecium</i> ST80-lineages by 70% of patients in an Irish hospital.

ABSTRACT:

Background

Vancomycin-resistant Enterococcus faecium (VREfm) are significant nosocomial pathogens. Irish VREfm comprise diverse vanA-encoding ST80-complex type (CT) lineages. Recent studies indicate that within-patient VREfm diversity could confound surveillance. This study investigated the intra-host VREfm genetic diversity among colonized Irish hospital patients.

Methods

Rectal VREfm (n = 150) from 10 patients (15 isolates each) were investigated by WGS, core-genome MLST and split k-mer (SKA)-SNP analysis. Plasmids and vanA-transposons from 39 VREfm representative of CTs identified were resolved by hybrid assembly of short-read (Illumina) and long-read (Oxford Nanopore Technologies) sequences. Plasmid relatedness was assessed based on Mas

SUBMITTER: Kavanagh NL 

PROVIDER: S-EPMC12039289 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature