Tobacco carcinogen NNK promotes pancreatic cancer proliferation via LINC00857/β-catenin.

ABSTRACT:

Introduction

Smoking is a key risk factor for pancreatic cancer (PC). Nicotine-derived nitrosamine ketone (NNK), a major tobacco smoke constituent, has been shown to promote cancer growth, but its specific role in PC progression remains unclear. While long non-coding RNA LINC00857 (lnc RNA) is implicated in cancer progression, its regulation by NNK is unknown. This study aims to investigate whether NNK can drive PC growth and elucidate the underlying mechanisms.

Methods

Employing an experimental methodology, this investigation treated human pancreatic cancer cell lines (CFPAC-1 and Panc-1) with NNK and utilized various assays (CCK-8, colony formation, and EdU cell proliferation) to assess the effects on cell proliferation. The interplay between LINC00857 expression profiles,

SUBMITTER: Zhou C 

PROVIDER: S-EPMC12039305 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature