Improving Glycoproteomic Analysis Workflow by Systematic Evaluation of Glycopeptide Enrichment, Quantification, Mass Spectrometry Approach, and Data Analysis Strategies.

ABSTRACT: Glycosylation is one of the most prevalent and crucial protein modifications. Quantitative site-specific characterization of glycosylation usually requires sophisticated intact glycopeptide analysis using glycoproteomics. Recent efforts have focused on the interrogation of intact glycopeptide analyses using tandem mass spectrometry. However, a systematic evaluation of the quantitative glycoproteomic workflow is still lacking. This study compared different strategies for glycopeptide enrichment alongside glycopeptide quantitation, as well as mass spectrometry and data analysis strategies, providing a comprehensive assessment of their efficacy. The ZIC-HILIC enrichment method demonstrated superior performance, representing a 26% improvement in identified glycopeptiudes compared to the MAX en

SUBMITTER: Sun Z 

PROVIDER: S-EPMC12039365 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature