High-performance protocol for ultra-short DNA sequencing using Oxford Nanopore Technology (ONT).

ABSTRACT: In recent years, Oxford Nanopore Technologies (ONT) has gained substantial attention across various domains of nucleic acid research, owing to its unique advantages over other sequencing platforms. Originally developed for long-read sequencing, ONT technology has evolved, with recent advancements enhancing its applicability beyond long reads to include short, synthetic DNA-based applications. However, sequencing short DNA fragments with nanopore technology often results in lower data quality, likely due to the absence of protocols optimised for these fragment sizes. To address this challenge, we refined the standard ONT library preparation protocol to improve its performance for ultra-short DNA targets. By utilising the same core reagents required for conventional ONT workflows, we introdu

SUBMITTER: Zemaitis L 

PROVIDER: S-EPMC12040124 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature