CAMP-independent Crp homolog adds to the multi-layer regulatory network in <i>Porphyromonas gingivalis</i>.

ABSTRACT:

Introduction

Porphyromonas gingivalis encodes three CRP/FNR superfamily proteins: HcpR, PgRsp, and Crp^Pg, with Crp^Pg similar to cAMP-sensing proteins but not classified into known families. This study investigates the role of Crp^Pg in regulating the expression of factors essential for P. gingivalis virulence in A7436 and ATCC 33277 strains.

Methods

The role of Crp^Pg protein in P. gingivalis was determined using the Δcrp^Pg mutant strains to characterize their phenotype and to assess the impact of crp^Pg inactivation on gene expression using RNA-seq and RT-qPCR. Additionally, the Crp^Pg protein was purified and characterized.

Results

Key findings in the Δcrp<

SUBMITTER: Smiga M 

PROVIDER: S-EPMC12040651 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature