Impact of DNA Extraction Methods on Gut Microbiome Profiles: A Comparative Metagenomic Study.

ABSTRACT: In gut microbial research, DNA extraction remarkably influences study outcomes and biological interpretations. Rapid advancements in the research scale and technological upgrades necessitate evaluating new methods to ensure reliability and precision in microbial community profiling. We systematically evaluated the performance of eight recent and commonly used extraction methods using a microbial mock community (MMC) and fecal samples from two healthy volunteers, incorporating bacterial, archaeal, and fungal constituents. Performance metrics included nucleic acid assessment, microbial profile assessment, and scalability for large-scale studies, leveraging shotgun metagenomics for in-depth analysis. Despite variations in DNA quantity and quality, all methods yielded sufficient DNA for shotgu

SUBMITTER: Pu Y 

PROVIDER: S-EPMC12040788 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature