Whole genome sequencing informs SNP-based breeding strategies to safeguard genetic diversity in captive African lions.

ABSTRACT:

Introduction

African lions (Panthera leo) face severe population declines, making captive breeding programs essential for conservation. However, genetic data scarcity in such programs elevates inbreeding risks and threatens genetic diversity.

Methods

Using next-generation sequencing (NGS), we analyzed genome-wide genetic markers from 10 captive African lions at Xinjiang Tianshan Wildlife Park. We identified high-confidence SNPs, evaluated population structure, and calculated kinship/inbreeding coefficients alongside identity by descent (IBD) and identity by state (IBS) analyses.

Results

We identified 5,051,795 high-confidence SNPs. The population contained distinct genetic subgroups. Six lion pairs exhibited elevated kinship coefficients, with one individual sh

SUBMITTER: Chen W 

PROVIDER: S-EPMC12040825 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature