Unveiling the potential impact of RNA m5C methyltransferases NSUN2 and NSUN6 on cellular aging.

ABSTRACT: NSUN2 and NSUN6, two family members of NOL1/NSUN protein, are mainly responsible for catalyzing the formation of 5-methylcytosine (m5C) in RNA and highly involved in the physiological and pathological processes of many diseases. To investigate the biological functions of NSUN2 and NSUN6, NSUN2^-/- and NSUN6^-/- HEK293T cell lines were separately constructed by CRISPR/Cas9. We found no significant interaction between the protein expression of NSUN2 and NSUN6. Notably, the ablation of NSUN2 or NSUN6 reduced cell proliferation and increased expression of the senescence-associated marker P27, whereas more β-galactosidase-positive cells were observed in response to H₂O₂-induced oxidative stress. Moreover, the expression of NSUN2 and NSUN6 was significan

SUBMITTER: Zhou J 

PROVIDER: S-EPMC12040966 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature