DNA content, repeatome composition and origin of the Zea mays micronuclei.

ABSTRACT: Micronuclei originate from DNA damage generated by clastogenic and/or by aneugenic effects. Depending on the pattern of damage, they may have distinct genomic origin and composition. Sequences of the centromere, telomere and rDNA have been identified in plant micronuclei. However, other DNA sequences may also be present in the micronuclei, as well as their DNA contents may be different. Here, we investigate the DNA content, genomic composition and origin of micronuclei induced in Zea mays by methyl methanesulfonate (MMS). DNA contents showed a wide range of distribution, suggesting their diverse genomic origins and illustrating how much of the nuclear genome can be lost due to mutagen effects. Micronuclei diversity was also evidenced by in situ probing with different DNA sequences (5S and

SUBMITTER: Tostes NV 

PROVIDER: S-EPMC12041367 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature