Phage-phage competition and biofilms affect interactions between two virulent bacteriophages and Pseudomonas aeruginosa.

ABSTRACT: Virulent bacteriophages (or phages) are viruses that specifically infect and lyse a bacterial host. When multiple phages co-infect a bacterial host, the extent of lysis and dynamics of bacteria-phage and phage-phage interactions are expected to vary. The objective of this study is to identify the factors influencing the interaction of two virulent phages with different Pseudomonas aeruginosa growth states (planktonic, an infected epithelial cell line, and biofilm) by measuring the bacterial time-kill and individual phage replication kinetics. A single administration of phages effectively reduced P. aeruginosa viability in planktonic conditions and infected human lung cell cultures, but phage-resistant variants subsequently emerged. In static biofilms, the phage combination displayed initia

SUBMITTER: Burkle M 

PROVIDER: S-EPMC12041424 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature