Analytical validation of total mucin concentration assay using SEC MALLS dRI for diagnosing and monitoring mucoobstructive lung diseases.

ABSTRACT: Mucins play a pivotal role in the pathophysiology of mucoobstructive lung diseases. Accurate quantification of total mucin concentrations in clinical sputum samples is critical for developing objective biomarkers for diagnosis, prognosis, and therapeutic monitoring. By using sputum samples and mucin standards, the analytical performance of the measurements of total mucin concentration by Size Exclusion Chromatography coupled with Multi-Angle Laser Light Scattering and Differential Refractometer [SEC-(MALLS)-dRI] method was assessed using universal validation metrics, including precision, accuracy, recovery, parallelism, specificity, linearity, and sample stability. Possible sample contamination sources, such as saliva, blood, and DNA, were also evaluated. The method demonstrated excellent

SUBMITTER: Ozkan E 

PROVIDER: S-EPMC12041582 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature