Isothermal Disorder-to-Order Transitions of DNA Origami Structures Induced by Alternative Component Subsets.

ABSTRACT: DNA origami technology has shown potential across various applications, including the construction of molecular machines. Among these, mimicking the complex structural transitions of natural biomolecules in physiological environments remains a long-standing pursuit. Here, inspired by intrinsically disordered proteins, we propose a strategy for inducing disorder-to-order transitions in DNA origami structures at room temperature using alternative component subsets. In a triangular DNA origami model, we define three subsets of its constitutional DNA staples based on their spatial distributions along the scaffold. Atomic force microscopy and molecular dynamics simulations show that the individual subsets result in metastable assemblies with disordered morphologies and elevated free-energy fluc

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12041949 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature