Fluorogenic Linkage Integration for Nonfluorescent Transformations (FLINT).

ABSTRACT: Since its creation, single-molecule optical imaging has revolutionized the study of catalytic processes, yet its application largely relies on probing fluorogenic reactions. To overcome this limitation, we propose the Fluorogenic Linkage Integration for Nonfluorescent Transformation (FLINT) approach, an imaging method to resolve nonfluorogenic reactions at the single-molecule level. Using glucose oxidation as a model reaction, we coupled this nonfluorogenic reaction with a fluorogenic Amplex Red (AR) → resorufin (RF) transformation to create a cascading reaction. This integration allowed us to monitor single-turnover events and extract key kinetic parameters for glucose oxidation despite their being invisible under the optical microscope. Our ensemble measurements combining cyclic voltamme

SUBMITTER: Pokhrel B 

PROVIDER: S-EPMC12042045 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature