Understanding the Relationship between Pressure and Temperature Unfolding of Proteins.

ABSTRACT: Proteins unfold under different environmental insults, among which are heat, cold, high pressure, and chaotropic agents. Understanding the mechanisms that determine unfolding under each of these conditions is an important problem that directly relates to the physical forces that determine the three-dimensional structure of a protein. Here, we studied a residue-specific description of the unfolding transitions of marginally stable yeast protein Yfh1 using high-pressure nuclear magnetic resonance. We compared the cold, heat, and pressure unfolded states and demonstrated what has up to now been only a hypothesis: the pressure-unfolded spectrum at room temperature shares features in common with that at low but not at high temperature and room pressure, suggesting a tighter similarity of the me

SUBMITTER: Roumestand C 

PROVIDER: S-EPMC12042054 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature