A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in <i>Mycobacterium abscessus</i>.

ABSTRACT: Mycobacterium abscessus, a fast-growing, non-tuberculous mycobacterium resistant to most antimicrobial drugs, causes a wide range of serious infections in humans, posing a significant public health challenge. The development of effective genetic manipulation tools for M. abscessus is still in progress, limiting both research and therapeutic advancements. However, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) systems have emerged as promising tools for generating highly specific double-strand breaks (DSBs) in its genome. One of the mechanisms that repair these DSBs is the error-prone nonhomologous end-joining (NHEJ) pathway, which facilitates targeted gene editing. In this study, we introduced a novel application of the CRISPR-

SUBMITTER: Zeng S 

PROVIDER: S-EPMC12042118 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature