The GntR/VanR transcription regulator AlkR represses AlkB2 monooxygenase expression and regulates <i>n</i>-alkane degradation in <i>Pseudomonas aeruginosa</i> SJTD-1.

ABSTRACT: Transmembrane alkane monooxygenase (AlkB)-type monooxygenases, especially AlkB2 monooxygenases, are crucial for aerobic degradation of the medium-to-long-chain n-alkanes in hydrocarbon-utilizing microorganisms. In this study, we identified a GntR/VanR transcription regulator AlkR of Pseudomonas aeruginosa SJTD-1 involved in the negative regulation of AlkB2 and deciphered its nature of DNA binding and ligand release. The deletion of alkR enhanced the transcription levels of the alkB2 gene and the utilization efficiency of the medium-to-long-chain n-alkanes by strain SJTD-1. The dimer of AlkR recognizes and binds to a conserved palindromic motif in the promoter of the alkB2 gene, and structural symmetry is vital for DNA binding and transcription repr

SUBMITTER: Peng W 

PROVIDER: S-EPMC12042122 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature