Introduction of a phenylalanine sink in fast growing cyanobacterium Synechococcus elongatus PCC 11801 leads to improved PSII efficiency, linear electron transport, and carbon fixation.

ABSTRACT: Cyanobacteria are investigated for fundamental photosynthesis research and sustainable production of valuable biochemicals. However, low product titer and biomass productivities are major bottlenecks to the economical scale-up. Recent studies have shown that the introduction of a metabolic sink, such as sucrose, 2,3-butanediol, and 2-phenyl ethanol, in cyanobacteria improves carbon fixation by relieving the "sink" limitation of photosynthesis. However, the impact of light intensity on the behavior of this sink-derived enhancement in carbon fixation is not well understood and is necessary for translation to outdoor cultivation. Here, using random mutagenesis, we engineered Synechococcus elongatus PCC 11801 to overproduce 1.24 g L^-1 phenylalanine (Phe) in 3 days, identified L531W

SUBMITTER: Deshpande A 

PROVIDER: S-EPMC12043352 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature