Comprehensive analysis of transcriptome and microbiome in colorectal cancer with synchronous polyp patients.

ABSTRACT:

Background

Colorectal cancer (CRC) is a prevalent and lethal malignancy, with the role of gut microbiota in its development still unclear. This study examines differences in gut microbiota between CRC patients and healthy controls and explores their association with host gene expression to identify potential diagnostic and therapeutic targets.

Methods

Fecal samples from 10 CRC patients and 13 healthy controls were subjected to 16S rRNA sequencing. Transcriptome sequencing of tumor tissues, normal mucosa, and colorectal polyps from same 10 CRC patients was performed to identify differentially expressed genes (DEGs). Pearson correlation analysis was employed to associate operational taxonomic units (OTUs) with host gene expression.

Results

β-diversity analysis showed si

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12043645 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature