Therapeutic mechanistic study of novel indole derivatives as SIRTUIN3 modulators in Parkinson's disease with in vitro evaluation.

ABSTRACT: SIRT3 modulates reactive oxygen species and helps to reduce oxidative stress, resulting in a neuroprotective effect in Parkinson's disease. The work seeks to develop and synthesize novel, effective SIRT3 modulators, which will modify SIRT3 expression and generate the neuroprotective effect by downregulating reactive oxygen species. The synthesis of the new indole-based carboxamide derivatives IMW 1-16 was guided by molecular docking experiments with the SIRT3 wild-type protein. FT-IR, ¹H-NMR, ¹³C-NMR, and mass spectrometry analyzed the synthesized compounds. The efficacy of the compounds IMW 1-16 was assessed using in-vitro assays such as the MTT assay, neuroprotection assay, lactate dehydrogenase assay, superoxide dismutase assay, glutathione peroxidase assay, reacti

SUBMITTER: Chand J 

PROVIDER: S-EPMC12044002 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature