A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue.

ABSTRACT: The tumor microenvironment comprises different cell subsets including myeloid-derived suppressor cells, which exert intratumoral immunosuppression and favor cancer progression. Isolating tumor-infiltrating myeloid-derived suppressor cells (tMDSCs) from human tumor samples remains a challenge. Current methods such as magnetic bead sorting (MACS) or flow cytometry sorting (FACS) present some drawbacks in terms of purity and viability. Here, we have setup an innovative workflow that combines RosetteSep technology and MACS for isolation of tMDSCs from lung cancer biopsies. To evaluate our Rosette-MACS approach, we compared its performance with MACS and FACS. The isolated cells were characterized by flow cytometry, gene expression analysis and proliferation assays for comparison purposes. The r

SUBMITTER: Piqueras-Nebot M 

PROVIDER: S-EPMC12044027 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature