Centrifugal Microfluidic Lateral Flow Assay Enables High Sensitivity Interleukin-6 Detection and Ultrafast Readout of Elevated Analyte Levels.

ABSTRACT: Balancing sensitivity and time to result is always a challenging part of the development of analytical tools and is of particular importance in clinical and point-of-care diagnostics. In this study, a highly sensitive fluorescence interleukin-6 lateral flow assay (LFA) was developed using centrifugal microfluidics. The sample flow rate through the lateral flow membrane is determined by centrifugal force, which can be precisely controlled with a processing device. Using this precise flow control, an ultrafast early readout after 30 s with a sensitivity of 78.3 pg/mL and a quantitative measurement up to 2000 pg/mL was achieved. Afterward, the flow rate was reduced, and thus, the incubation time increased to achieve a maximum sensitivity of 1.2 pg/mL within 13 min of run time. This high-perfo

SUBMITTER: Kainz DM 

PROVIDER: S-EPMC12044588 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature