Cancellation of Spectral and Spatial Crosstalk in Spectral Imaging for High-Dynamic-Range Electrophoretic Analysis of STR-PCR Products.

ABSTRACT: In spectral imaging, an optical system generates two mutually dependent kinds of crosstalk on an image sensor: spectral crosstalk (i.e., spectral mixing) between fluorescences of different dyes and spatial crosstalk (i.e., image artifacts) between fluorescences from different emission points in the field of view of the sensor. Therefore, an algorithm to cancel both kinds of crosstalk simultaneously (i.e., simultaneous spectral unmixing and image-artifact reduction) is proposed. The algorithm is based on the assumption that a crosstalk matrix (i.e., point-spread functions, PSFs) consisting of all crosstalk ratios is constant regardless of the causes of both kinds of crosstalk. By applying the algorithm to a nine-wavelength-band measurement of four-capillary electrophoretic separation of STR

SUBMITTER: Anazawa T 

PROVIDER: S-EPMC12044589 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature