Assessment of Biotransformation Rates, Biomagnification, and Bioconcentration Factors of Difficult-to-Test Substances Using In Vitro Rainbow Trout Liver Subcellular Fractions.

ABSTRACT: In vitro hepatic biotransformation assays have been proposed as methods for expediting and improving bioaccumulation assessment for substances that are difficult to test in conventional in vivo bioaccumulation assays and to reduce animal testing. However, to date, there has been no convincing evidence to demonstrate the ability and limitations of in vitro assays to estimate in vivo biotransformation rates and corresponding bioconcentration and biomagnification factors of difficult-to-test substances in fish. The present study presents the first measurements of in vitro biotransformation rates of superhydrophobic and highly volatile linear and cyclic siloxanes in rainbow trout liver S9 subcellular fractions and the extrapolation of in vitro rates to whole body in vivo biotransformation rate

SUBMITTER: Cantu MA 

PROVIDER: S-EPMC12044689 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature