Lung-directed delivery of a ligand-mediated chimeric lysin has an enhanced ability to eradicate pulmonary and intracellular Staphylococcus aureus.

ABSTRACT:

Background

Bacteriophage lysins have high antimicrobial activities with many advantages as alternatives to antibiotics, however, lysins generally do not exhibit intracellular bactericidal capabilities due to a lack of cell-penetrating properties and/or reduced activity under the intracellular environment. To address this problem, p-ClyC, an engineered chimeric lysin with a lung cell-targeting peptide, was used to kill Staphylococcus aureus (S. aureus) in vitro and in vivo.

Methods

p-ClyC was constructed by fusing ClyC with a lung-directed peptide. Antimicrobial activities of the two lysins (ClyC, p-ClyC) against S. aureus were evaluated in vitro and in a murine lung infection model. The cell internalization of the lysins was explored using laser confocal imaging. The intrace

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12044732 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature