Quantitative stable isotope probing (qSIP) and cross-domain networks reveal bacterial-fungal interactions in the hyphosphere.

ABSTRACT:

Background

Interactions between fungi and bacteria have the potential to substantially influence soil carbon dynamics in soil, but we have yet to fully identify these interactions and partners in their natural environment. In this study, we stacked two powerful methods, ¹³C quantitative stable isotope probing (qSIP) and cross-domain co-occurrence network, to identify interacting fungi and bacteria in a California grassland soil. We used in-field whole plant ¹³CO₂ labeling along with sand-filled ingrowth bags (that trap fungi and hyphae-associated bacteria) to amplify the signal of fungal-bacterial interactions, separate from the bulk soil background.

Results

We found a total of 54 bacterial ASVs and 9 fungal OTUs that were significantly

SUBMITTER: Slanzon GS 

PROVIDER: S-EPMC12044819 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature