Harnessing the angiogenic potential of adipose-derived stromal vascular fraction cells with perfusion cell seeding.

ABSTRACT:

Background

The rapid formation and long-term maintenance of functional vascular networks are crucial for the success of regenerative therapies. The stromal vascular fraction (SVF) from human adipose tissue is a readily available, heterogeneous cell source containing myeloid lineage cells, mesenchymal stromal cells, endothelial cells and their precursors, and pericytes, which are important for vascular support. Previous studies showed that seeding SVF cells under perfusion and pre-culturing them on three-dimensional (3D) collagen sponges enhances the vascular cell component in vitro while accelerating vascularization and improving human cell engraftment in vivo compared to static pre-culture. However, generating a perfusion-cultured SVF patch over a 5-day period is both costly and c

SUBMITTER: Reid G 

PROVIDER: S-EPMC12044990 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature