Global landscape of protein phosphorylation during plant regeneration initiation in cotton (Gossypium hirsutum L.).

ABSTRACT:

Background

Phosphorylation is one of the most common post-translational modifications and is central to many cellular signaling events; however, little is currently known about the phosphorylation landscape during somatic embryogenesis (SE) for plant regeneration.

Results

Here, we systematically analyzed the phosphoproteomic profile of three typical developmentally staged cultures of SE, non-embryogenic calli (NEC), primary embryogenic calli (PEC), and globular embryos (GE), in cotton (Gossypium hirsutum L.), the pioneer crop for genetic biotechnology applications. Our data revealed a total of 6301 quantifiable phosphorylation sites in 2627 quantifiable phosphoproteins from 5548 modified peptides, of which 1105 phosphoproteins (2147 sites) were differentially phosphorylated.

SUBMITTER: Guo H 

PROVIDER: S-EPMC12044994 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature