Compound screening in human airway basal cells identifies Wnt pathway activators as potential pro-regenerative therapies.

ABSTRACT: Regeneration of the airway epithelium restores barrier function and mucociliary clearance following lung injury and infection. The mechanisms regulating the proliferation and differentiation of tissue-resident airway basal stem cells remain incompletely understood. To identify compounds that promote human airway basal cell proliferation, we performed phenotype-based compound screening of 1429 compounds (from the ENZO and Prestwick Chemical libraries) in 384-well format using primary cells transduced with lentiviral luciferase. A total of 17 pro-proliferative compounds were validated in independent donor cell cultures, including the antiretroviral therapy agent abacavir and several Wnt signalling pathway-activating compounds. The effects of compounds on proliferation were further explored i

SUBMITTER: Ishii Y 

PROVIDER: S-EPMC12045047 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature