AZGP1 Attenuates Subretinal Fibrosis and Inhibits Epithelial-Mesenchymal Transition by Blocking the PI3K/AKT Signaling Pathway.

ABSTRACT:

Purpose

Subretinal fibrosis (SRF) represents a significant contributor to irreversible vision loss in patients with neovascular age-related macular degeneration (nAMD). This study aimed to elucidate the underlying mechanism of SRF and identify potential therapeutic targets.

Methods

The SRF model was established using a two-stage laser-induced protocol in C57BL/6J mice. RNA-seq analysis was conducted to identify differentially expressed genes (DEGs) at 10 days and 30 days post-second laser. Quantitative RT-PCR was used to validate the expression levels of selected DEGs including zinc-alpha-2-glycoprotein 1 (AZGP1). Recombinant AZGP1 (rAZGP1) was intravitreally administrated to investigate its effects on SRF. The ARPE-19 cells were used to demonstrate the role of AZGP1 in modu

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12045119 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature