Metagenomic investigation of bacterial laccases in a straw-amended soil.

ABSTRACT:

Background

Bacterial laccases play a crucial role in the degradation of lignin and the turnover of soil organic matter. Their advantageous properties make them highly suitable for a wide range of industrial applications. However, the limited identification of these potential enzymes has impeded their full utilization. The straw-amended soil provides materials for the development of bacterial laccases.

Methods

Metagenomic sequencing of a straw-amended soil was conducted to explore novel bacterial laccases. The putative bacterial laccases were then screened using profile hidden Markov models for further analysis. The most abundant gene, lacS1, was heterologously expressed in Escherichia coli and the recombinant laccase was purified for enzymatic characterization.

SUBMITTER: Yu D 

PROVIDER: S-EPMC12045287 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature