Single-molecule nanopore sensing of proline <i>cis</i>/<i>trans</i> amide isomers.

ABSTRACT: Molecules known as stereoisomers possess identical numbers and types of atoms, which are oriented differently in space. Cis-trans isomerization of proline, a distinctive case of stereoisomerism in peptides and proteins, includes the rearrangement of chemical groups around an acyl-proline amide bond that bears the partial double bond character. Many cellular processes are affected by cis-trans proline isomerization and associated conformational protein interconversions. This work explored the conformer ratio of natural and chemically modified prolines using the aerolysin pore as a nanosensor. Despite the well-known involvement of proline in protein folding, stability, and aggregation, the highly demanding discrimination of cis and trans isomers of t

SUBMITTER: Iesu L 

PROVIDER: S-EPMC12045290 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature