Nucleic acid sequence determinants of transcriptional pausing by human mitochondrial RNA polymerase (POLRMT).

ABSTRACT: Transcription by RNA polymerase (RNAP) lies at the heart of gene expression in all organisms. The speed with which RNAPs produce the RNA is tuned in part by the signals in the transcribed nucleic-acid sequences, which temporarily arrange RNAPs into a paused conformation unable to extend the RNA. In turn, the altered transcription kinetics determines the three-dimensional shape into which RNA ultimately folds, dictates the chromatin state, and promotes or inhibits co-transcriptional events. While pause sequence determinants have been characterized for multi-subunit RNAPs in bacteria and the eukaryotic nuclei, this information is lacking for the single-subunit RNAP of human mitochondria, POLRMT. Here, we developed a robust nucleic-acid scaffold system to reconstitute POLRMT transcription

SUBMITTER: Hsieh AH 

PROVIDER: S-EPMC12045343 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature