Improved DNA recovery and STR profile development from weathered Bos taurus bones using demineralized bone slices.

ABSTRACT: Current processing techniques for harvesting DNA from osseous elements are destructive, and success rates vary widely. When skeletal elements are homogenized into a fine powder, endogenous DNA may be subjected to fragmentation, and the likelihood of introducing co-purified inhibitory substances to the sample increases. While a limited number of articles in the relevant literature have challenged the status quo of pulverization, powdering hard tissue samples before DNA isolation continues to be standard practice in the forensic and ancient DNA communities. In this work, we have developed and optimized an alternative front-end processing method for demineralizing and slicing cortical bone using aged and weathered bovine skeletal samples as a model for degraded human bone. Additionally, this

SUBMITTER: Rhodes C 

PROVIDER: S-EPMC12046109 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature