In Vivo Quantification of Creatine Kinase Kinetics in Mouse Brain Using ³¹P-MRS at 7 T.

ABSTRACT: ³¹P-MRS is a method of choice for studying neuroenergetics in vivo, but its application in the mouse brain has been limited, often restricted to ultrahigh field (> 7 T) MRI scanners. Establishing its feasibility on more readily available preclinical 7-T scanners would create new opportunities to study metabolism and physiology in murine models of brain disorders. Here, we demonstrate that the apparent forward rate constant (k_f) of creatine kinase (CK) can be accurately quantified using a progressive saturation-transfer approach in the mouse brain at 7 T. We also find that a 20% reduction in respiration of anesthetized mice can lead to 36% increase in k_f attributable to a drop in cellular pH and mitochondrial ATP production. To achieve this, we used a test-r

SUBMITTER: Tachrount M 

PROVIDER: S-EPMC12046211 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature