In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector.

ABSTRACT:

Background

Goose astrovirus (GAstV) is an emerging pathogen that is widely distributed throughout China and can cause visceral gout, resulting in serious economic losses for the goose industry. Open reading frame 2 (ORF2) of this virus encodes the precursor capsid protein, which is essential for the assembly and antigenicity of these virions. To construct a bi-valent vaccine for controlling GAstV and duck enteritis virus (DEV) infection, an infectious bacterial artificial chromosome (BAC) clone of the DEV vaccine strain pDEV-EF1 was used to establish a recombinant DEV vector for GAstV ORF2 gene delivery.

Methods

GAstV ORF2 expression frame was inserted into the US7 and US8 intergenic region of DEV genome by Red E/T two-step recombinant technology, then the recombinant virus

SUBMITTER: Chen L 

PROVIDER: S-EPMC12046716 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature