Advances in the study of extracellular vesicles of Naegleria fowleri and their role in contact-independent pathogenic mechanisms.

ABSTRACT:

Background

Extracellular vesicles (EVs) are spherical membrane particles released by prokaryotic and eukaryotic cells. EVs produced by pathogenic organisms are known to play a role in host-pathogen interactions; however, despite some reports on Naegleria fowleri EVs, their potential role in inducing cytopathic effects remains poorly understood. In this study, we evaluated the role of N. fowleri EVs in contact-independent pathogenic mechanisms.

Methods

Extracellular vesicles were characterized via transmission electron microscopy, nanoparticle tracking analysis, SDS-PAGE, mass spectrometry, Western blotting, and zymography. EVs internalization by trophozoites and MDCK epithelial cells was also determined. Finally, mammalian cells were coincubated with EVs to evaluate haemolyt

SUBMITTER: Castelan-Ramirez I 

PROVIDER: S-EPMC12046931 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature