Spatial differences in gene expression across the dorsal raphe nucleus in a model of early Alzheimer's disease.

ABSTRACT:

Background

Persons with Alzheimer's disease (AD) present with changes in mood, sleep, and arousal that may precede the clinical manifestation of cognitive decline. These early symptoms can be driven by changes in the serotonergic (5-HT) nuclei of the brainstem, particularly the dorsal raphe nucleus (DRN). It is unclear why all 5-HT neurons do not simultaneously develop AD pathology that progresses at the same rate.

Objective

We sought to identify any underlying genetic components associated with susceptibility or resistance of 5-HT neurons to AD pathology.

Methods

The Visium Spatial Gene Expression platform was used to identify transcriptomic changes across the DRN in a preclinical model of early AD, human tau-overexpressing mice (htau mice). We further used RNAscope

SUBMITTER: Kolling LJ 

PROVIDER: S-EPMC12047055 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature