Understanding the role of vascular stretch on modulation of VWF and ANGPT-2 in continuous flow left ventricular assist device (CF-VAD) patients.

ABSTRACT: Non-surgical bleeding is a common complication in patients on continuous flow left ventricular assist device (CF-VAD) support. This study investigates how the transition from cyclic to constant stretch following CF-VAD implantation affects endothelial biosynthesis and release of Von Willebrand factor (VWF) and angiopoietin-2 (ANGPT-2), two molecules that play an essential role in the development of non-surgical bleeding. Human aortic endothelial and umbilical vein endothelial cells (HAECs and HUVECs) were cultured within a uniaxial stretch device that mimics stretch associated with both normal pulsatile and CF-VAD conditions. Following 72 hours of stretch, transcriptional regulation, intracellular accumulation, and secretion of VWF and ANGPT-2 were evaluated using molecular expression prof

SUBMITTER: Sah JP 

PROVIDER: S-EPMC12047207 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature