De novo antibody identification in human blood from full-length single B cell transcriptomics and matching haplotype-resolved germline assemblies.

ABSTRACT: Immunoglobulin (IGH, IGK, IGL) loci in the human genome are highly polymorphic regions that encode the building blocks of the light and heavy chain IG proteins that dimerize to form antibodies. The processes of V(D)J recombination and somatic hypermutation in B cells are responsible for creating an enormous reservoir of highly specific antibodies capable of binding a vast array of possible antigens. However, the antibody repertoire is fundamentally limited by the set of variable (V), diversity (D), and joining (J) alleles present in the germline IG loci. To better understand how the germline IG haplotypes contribute to the expressed antibody repertoire, we combined genome sequencing of the germline IG loci with single-cell transcriptome sequencing of B cells from the s

SUBMITTER: Beaulaurier J 

PROVIDER: S-EPMC12047243 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature