Engineering a custom-sized DNA scaffold for more efficient DNA origami-based nucleic acid data storage.

ABSTRACT: DNA has emerged as a promising material to address growing data storage demands. We recently demonstrated a structure-based DNA data storage approach where DNA probes are spatially oriented on the surface of DNA origami and decoded using DNA-PAINT. In this approach, larger origami structures could improve the efficiency of reading and writing data. However, larger origami require long single-stranded DNA scaffolds that are not commonly available. Here, we report the engineering of a novel longer DNA scaffold designed to produce a larger rectangle origami needed to expand the origami-based digital nucleic acid memory (dNAM) approach. We confirmed that this scaffold self-assembled into the correct origami platform and correctly positioned DNA data strands using atomic force microscopy and DN

SUBMITTER: Kobernat SE 

PROVIDER: S-EPMC12047451 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature