Expanding kinetoplastid genome annotation through protein structure comparison.

ABSTRACT: Kinetoplastids belong to the Discoba supergroup, an early divergent eukaryotic clade. Although the amount of genomic information on these parasites has grown substantially, assigning gene functions through traditional sequence-based homology methods remains challenging. Recently, significant advancements have been made in in-silico protein structure prediction and algorithms for rapid and precise large-scale protein structure comparisons. In this work, we developed a protein structure-based homology search pipeline (ASC, Annotation by Structural Comparisons) and applied it to transfer biological information to all kinetoplastid proteins available in TriTrypDB, the reference database for this lineage. Our pipeline enabled the assignment of structural similarity to a substantial portion of k

SUBMITTER: Trinidad-Barnech JM 

PROVIDER: S-EPMC12047770 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature